LBSD-X SOLUBILISATION BUFFER AND PSEUDO-VIRUS SARS-COV2 POSITIVE CONTROL TESTING KIT

£199.00

SARS-CoV2 pseudo-virus positive control expressing Spike protein

Dissolution and Solubilisation LSBDS-X Buffer, MS-compatible

One mL of 10x buffer & 300μl of positive control included

Reconstitute and dilute prior to use

 

About

The kit comprises of two items; the Dissolution and Solubilisation Buffer X (LBSD-X) and Pseudo Virus expressing SARS-CoV-2 Spike protein grown in culture HEK293T/17 cells. Spike proteolytic fragments S1 and S2 were seen in all preparations. Buffer is conveniently stored at room temperature and the positive control is refrigerated. LBSD-X is supplied 10 x concentration and is required to be diluted prior to use. Positive control for SARS-CoV2 is delivered in a freeze-dried powder format and will be reconstituted prior to use.

Application:

The kit is specifically designed for researchers undertaking analysis for the SARS-CoV2 virus. It has been originally devised for application on MALDI-ToF Mass spectrometry, therefore, it is ionisation friendly.

Original research

The kit was originally developed to assist in the development of the MALDI-ToF mass spectrometry-based test for COVID-19 causing virus – SARS-CoV2.

Pseudo-virus constructs are ideal non-infectious starting material upon which to develop viral protein mass spectrometry tests. The pseudo-virus construct is based on the SARS-CoV2 Wuhan variant. Spike protein is fully expressed in the human cell line, HEK293; so that fully post-translationally modified, membrane-embedded viral proteins are expressed and tertiary structures are functionally intact.

The LBSD-X buffer is widely applicable for the research of all enveloped viruses. It is designed to be utilised with mass spectrometry methodology with the main purpose of membrane dissolution and viral envelope protein solubilization. Buffer’s intended purpose is to enable and enhance the research of the viral particles, which could be translated into clinical applications.

 

Further details of the original research could be found here.

Independent validation, at the University of Northern Illinois, using the provided kit materials could be found here.

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