SARS-CoV2 pseudo-virus positive control expressing Spike protein
Dissolution and Solubilisation LSBDS-X Buffer, MS-compatible
One mL of 10x buffer & 300μl of positive control included
Reconstitute and dilute prior to use
The kit was originally developed to assist in the development of the MALDI-ToF mass spectrometry-based test for COVID-19 causing virus – SARS-CoV2.
Pseudo-virus constructs are ideal non-infectious starting material upon which to develop viral protein mass spectrometry tests. The pseudo-virus construct is based on the SARS-CoV2 Wuhan variant. Spike protein is fully expressed in the human cell line, HEK293; so that fully post-translationally modified, membrane-embedded viral proteins are expressed and tertiary structures are functionally intact.
The LBSD-X buffer is widely applicable for the research of all enveloped viruses. It is designed to be utilised with mass spectrometry methodology with the main purpose of membrane dissolution and viral envelope protein solubilization. Buffer’s intended purpose is to enable and enhance the research of the viral particles, which could be translated into clinical applications.
Further details of the original research could be found here.
Independent validation, at the University of Northern Illinois, using the provided kit materials could be found here.
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